Research Technician II

Fred Hutchinson Cancer Research Center Shoreline, WA
Overview

Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch's pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation's first and largest cancer prevention research program, as well as the clinical coordinating center of the Women's Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.

The Greenberg group is part of a SU2C/AACR-funded multicenter "Dream Team" that is performing a T cell therapy trial for patients with non-small cell lung cancer or mesothelioma. This trial is testing the therapeutic activity of autologous CD8+ T cells, derived from either naïve or central memory T cell precursors, transduced to express a high affinity, HLA-A2-restricted, WT1-specific TCR developed in the our lab. This TCR is also being evaluated in a similar trial design for therapy of acute myelogenous leukemia (AML), a setting in which we have shown that WT-1 specific CD8 T cells can prevent leukemic relapse in at least a fraction of patients. We are also working to identify epitopes in WT1 and other antigens that are commonly found on AML and other malignancies and restricted to Class I MHC alleles beyond HLA-A2, so that TCRs can be isolated and high avidity CD8+ T cells generated to treat a much broader range of patients.

We are looking for a highly motivated individual to support the development of immunotherapy for cancer. The successful candidate will utilize a variety of cutting-edge cellular and molecular techniques to analyze T cell immunity to cancer. The technician will be closely involved in the preclinical development of new TCR-based therapies and their translation into clinical trials. The candidate should be capable of maintaining detailed laboratory records, analyzing data, and reporting experimental results.

Responsibilities

Assist in the design, implementation and interpretation of experiments involving human T lymphocytes engineered to target tumor cells. These studies are specifically aimed at identifying ways to increase the therapeutic activity of tumor-reactive T lymphocytes.

* Perform tissue culture and molecular biology techniques: Generation of antigen-specific T cells, transduction with retroviral and lentiviral vectors, FACS sorting and expanding cells in vitro.

* Analyze T cell phenotype and function using techniques including flow cytometry, proliferation assays, PCR, RT-PCR, sequencing, and T cell cytolytic assays.

* Some murine work may be necessary in the future, with the assistance of dedicated murine technicians

* Assist with the management and organization of a fast-paced lab. Other duties as assigned by supervisor will be performed as necessary including conducting literature reviews to assist in determining most suitable methods to be used in research.

* Contribute to the preparation and presentation of written or oral progress reports concerning studies and assist in publication of results.

Qualifications

Minimum Qualifications:

* Tech II level: A bachelor's degree in a biological field and two years or more of post-bachelor's laboratory research experience

* To succeed in this position candidate must enjoy working independently but be able to interact well in a team environment.

* Excellent organizational and interpersonal skills, the ability to multi-task, and attention to detail are critical.

Preferred:

* Immunology background with experience in aseptic cell culture techniques, multi-color flow cytometry and in establishing and maintaining primary human T cells is preferred.

* Molecular biology experience, retroviral/lentiviral transduction.

* Master's degree providing immunology training and/or relevant technical expertise a plus.

* Computer skills: Microsoft Excel, Prism (Graphpad), FlowJo and statistical programs for data analysis.

Our Commitment to DiversityWe are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We are proud to be an Equal Opportunity and VEVRAA Employer. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability, marital or veteran status, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at escmail@fredhutch.org or by calling 206-667-4700.

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